Immunohistochemical Detection Systems

Immunohistochemistry

Immunocytochemistry (or Immunohistochemistry ) is a laboratory technique that is used to anatomically study the distribution and contents of some certain chemical compoents of samples Using labeled specific antibody. The samples include tissue sections and cell specimens.

Principle

Immunohistochemistry is based on the principle of antigen-antibody reaction and chemical color development. The antigen in the tissue section or cell sample is first bound to the primary antibody, and the primary antibody is reacted with the labeled biotinylated secondary antibody, which is then labeled with horseradish peroxidase (HRP) or alkaline phosphatase (AKP), such as avidin (eg. streptavidin), and finally through the color reaction or fluorescence to show the chemical composition of cells or tissues, under the light microscope can clearly see the antigen-antibody reaction products occurring in the cells, thereby The ability to determine the distribution and content of certain chemical components in situ on cell slides or tissue sections.

After the enzyme-labeled antibody interacts with the tissue or cells, the substrate of the enzyme is added to generate a colored insoluble product or a particle having a certain electron density, and the surface antigen and various antigen components in the cell are localized by light microscopy or electron microscopy.

Immunoblotting technology is currently most commonly used. Compared with immunofluorescence technology, the main advantages of enzyme labeling method are accurate positioning, good contrast, and long-term preservation of stained specimens, suitable for light and electron microscope research.