Advantages |
Disadvantages |
System is not an organism and is not restricted by the policies imposed on genetically modified organisms (GMO) |
Short expression lifespan because of the limited energy of the system even in the presence of an ATP regenerating system |
Process is quick and simple requiring only preparation of cell extract and feeding solution and subsequent addition of DNA template |
Expensive system has no sustained metabolism to convert cheap energy (like sugars) into useable one for the gene expression machinery |
No concurrent expression of existing genome, therefore your genetically engineered device is more energy efficient |
Less characterization and experience of use in the laboratories compared to E. coli |
No DNA mutation of your genetically engineered device because there is no DNA replication |
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No selective pressure on your genetically engineered device because the system is non-living and does not undergo natural selection |
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No self-replication of your genetically engineered device leads to a fixed amount of DNA being expressed and more control over the rate of expression |
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Expression system can be quality-controlled by manipulating adjustable parameters e.g. buffers are added to maintain optimum magnesium concentrations for efficient translation; protease inhibitors can be added to minimize degradation of synthesized proteins |
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