Research programs with successful results depend on the use of sensitive and cost-saving antibodies with excellent performance. However, with millions of antibodies supplied by worldwide suppliers, finding the right antibodies seems to be a difficult task. To decrease the risk of failure, it’s quite worth taking time to search the right antibodies from a reliable supplier before a project or an experiment. How to choose the most suitable antibody reagents for your experiments? Leading Biology, committed to accelerating the lab high-productivity for researchers, here shares you with some useful tips to find the right reagents that ensure the generation of accurate results. 

Choose an antibody with a scientific manner.

We know that a professional experiment should be done with the scientific method that helps you plan, predict, research, conclude and maybe even publish your findings. Choosing your proper antibody also needs the scientific method to help your experiment more organized. To make a list of the details according to your experiment before you start the searching of antibodies. For example, the region of the protein that you wish to detect, what antigen you’d like to look for, what application you’d like to perform, etc. Be sure that the antibody should be suitable for your application. An antibody that works for one application will not be possible to work in every project. The nature of your sample will determine which antibody is most appropriate. 

When everything is confirmed, you can start searching. In order to help you make it easier, the website of Leading Biology provides the Search Function, which enables you to get the precise matching results from thousands of antibodies with a quick search and get the one you want right away.

Select an appropriate antibody host specie. 

Antibodies are frequently generated for use in research. As an antibody may react with the same target protein from other species sharing sufficient amino acid sequence homology, it’s suggested to choose an antibody against the same species that your sample is from with rigorous validation in order to avoid cross-reactivity and generation of misleading results.

Leading Biology offers primary antibodies that are produced as monoclonal (mAbs) or polyclonal antibodies (pAbs) using animal species such as the rat, mouse, goat, and rabbit as hosts. If the sample is from one of the species, this means that the species can be demonstrated suitability. mAbs are ideal for identical production and standardization experiments and produce minimal or no background staining. There is also a reduced possibility of cross-reaction with undesired proteins while using a mAb. Disadvantages may include unsuitability for antigen detection across multiple species, and vulnerability to epitope loss due to sample treatment methods. Whereas pAbs can bind to multiple epitopes of a single antigen, they are capable of recognizing even minor variations in the antigen. They also yield a stronger antigen detection signal. Hence they are ideal for experiments involving denatured proteins. Yet they may also be associated with unwanted background staining and batch-to-batch variability. Therefore, it’s important to choose the proper antibody from the host species.

Conduct the Background.

CiteAb is a professional platform for your reference and helps you to find out for determining an antibody in which applications are used and in which experimental conditions it has been got successful results. As it provides details that cite the use of its antibodies, it’s greatly suggested to conduct the background research on it for references before you make the final decision. All of Leading Biology primary and secondary antibodies have been certified for the high quality and could be found on the platform.

Don’t hesitate to contact us if any questions.

Antibodies play a fundamental role in the research and the selection of the right product can have a huge impact on the experiment results. The technical team of Leading Biology is always ready to provide support for you with antibody selection or any issues you might encounter in your experiment. Don’t hesitate to ask for help if you have any need.

Reviewing the protocols for optimal results

When choosing an antibody, be sure to review the protocols before you make the final decision, which can help you ensure that the product to be selected is compatible with your project. As most of the immunostaining protocols require careful optimization, there are several vital factors to be considered, for example, the nature of the blocking buffer, antibody diluents and wash solutions, the length of any incubation steps, and the method of detection. Some primary antibodies produce clear staining after a short incubation at room temperature, while others perform better with an overnight incubation at 4℃. Sample preparation is also important, with some antibodies recognizing an epitope in its native state and others binding only when the sample has been denatured. By studying the protocols provided by the manufacturer as well as researching the literature, the desired pattern of staining should be achievable.