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Location: Home > Information Center > FAQs > What are the procedures and steps followed in ELISA?

What are the procedures and steps followed in ELISA?

Date: 2020-08-05 Author: Leading Biology Click: 480

1. Antibody coating. The specific capture antibody is immobilized on high protein-binding plates by overnight incubation.

2. Protein capture. Samples and standard dilutions are added to the wells and will be captured by the bound antibodies.

3. Detection antibody.

4. Streptavidin-enzyme conjugate.

5. Addition of substrate.

6. Analysis.

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