PCR cleanup:
Leading Biology PCR kits can be used to remove all of the unwanted elements from your template. The growth media you use can also affect the outcome yields, while LB is usually fine. Sequencers are able to handle a wide range of DNA concentrations. The goal of purifying products before sequencing is to remove the excess primers and preserve the ratio of the dNTP to ddNTP, which is necessary for efficient sequencing reactions. Classical methods used to clean up PCR products before sequencing include gel electrophoresis, ethanol precipitation, and column chromatography. But these methods can be not applicable when processing multiple samples. If more than one PCR product is present, column purification, ethanol precipitation, or enzymatic purification will not isolate the desired product. So it’s suggested to use gel purification to isolate the desired product or reoptimize the PCR to obtain a single product.
Leading Biology Inc.
2600 Hilltop DR, Building G, B Suite C138
Richmond, CA, 94806
Tel: 1-661-524(LBI)-0262
Email: info@leadingbiology.com
