Can PCR primers be used for sequencing?_FAQs_Information Center

A PCR primer can be used for sequencing as long as it matches the conditions. In the process of PCR, copying segments of DNA whereby millions of copies of DNA can be generated from just a small sample. The process involves the use of primers, which are short strands of DNA generally about 15-30 nucleotides long. Two primers are used in each PCR reaction, and they are designed so that they flank the target region of interest (a region that should be copied). As the primers are custom-designed, they can be made up of many different nucleotide sequences, from which researchers can choose one that best fits their needs.

Be sure that excess PCR primers and unincorporated nucleotides should be removed before sequencing is started. Since one primer is used in each sequencing reaction, while 2 primers are used in PCR, if the 2 primers are not removed in PCR, you will get two sequences superimposed on each other that are not readable.

To obtain high quality sequencing data, it is very important to ensure a strong PCR reaction. Primers for PCR sequencing should have a GC content between 40% and 60%. The GC content (the number of G's and C's in the primer as a percentage of the total bases) of primer should be 40-60%. With Leading Biology PCR platform, which supports almost all of the reagents needed to run PCR testing, sequencing becomes more productive and cost-saving.

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Can PCR primers be used for sequencing?

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