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Location: Home > Information Center > Technical FAQs > Protein Technology Column > What are upstream technologies/approaches for enhancing prot

What are upstream technologies/approaches for enhancing prot

Date: 2020-04-15 Author: Leading Biology Click: 527

Several optimization strategies we commonly use are:
1/ Change the promoter to make the vector sequence more suitable for E. coli expression system.
2/ Try to insert genes into the E.coli's(or other expression system) genome to increase expression yield.
3/ Use a variety of BL21 strains (especially modified strains) to express proteins, such as some deleted protease genes' subtype.

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