What are some technical disadvantages of CRISPR/Cas9 and genetic engineering on the whole?

Disadvantages of CRISPR technology: CRISPR-Cas9 off-target: The effect of off-target can alter the function of a gene and may result in genomic instability, hindering it prospective and application in clinical procedure. A Single guide RNA also known as chimeric RNA is the combination of CRISPR RNA (CrRNA) and trans-activating RNA (TrRNA). SgRNA’s 20 base nucleotides are complimentary to the target DNA of interest with a trinucleotide known as Protospacer adjacent motif (PAM) adjacent to sequence which is mostly NGG (where N can be Adenine, Guanine, Cytosine or Thymine). Other studies reported that the PAM can also be NRG (where R is Adenine or Guanine). Even though DNA complimentary is highly specific based on base pair rule (A-T and G-C) there is still occurrence of off-target with 3 to 5 mismatches within the distal part of the PAM. SgRNA 5^' X20 PAM- 3^' (5^' X20 NGG-3^') where X20 is CrRNA contain both non-seed and seed sequences with many studies reporting that the seed sequence (mainly made up of 10-12 base pairs adjacent to the PAM) control the specificity of RNA guided endonucleases (RGens) and are regarded as more significance than the non-seed sequences.

Inquired items

Inquired items

All Departments

What are some technical disadvantages of CRISPR/Cas9 and gen

What are some technical disadvantages of CRISPR/Cas9 and genetic engineering on the whole?

Online Inquiry