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Location: Home > Custom Services > Protein Related Services > Virus-like particles/Phage library expression system based

Virus-like particles/Phage library expression system based

Date: 2018-08-28 Author: Leading Biology Click: 1767

Introduction

The so-called virus-like particles (VLP) is the artificial nanostructures produced by the virus gene modification, this kind of nanostructures could express the target protein. The VLP has self-assembly characteristics of a true virus, but it loses the Infectious ability of the virus. The gene that encodes the virus structural protein and the gene that encodes the target protein were fused together by using molecular biology technique, and the gene then transfected to a suitable host cell. The chimeric protein produced by this technique can be automatically assembled into a virus, and the target protein is expressed on the capsid of the virus.

VLP  

The insect cell system has been proven to be a powerful system for rapidly and easily producing VLPs, due to several convenient features, including its versatility and the rapid lead time needed for the construction of recombinant baculovirus. Besides that, insect cells system has other prominent advantages such as the high growth rate, the capacity for large-scale production, and the ability of several post-translational modifications similar to mammalian cells system. The major limitation of the insect cell system is the possible contamination of the enveloped baculovirus particles. To solve this problem, we have developed non-replicative baculovirus which reduces pollution to the minimum degree. In addition, we tested the silkworm expression system for VLPs production, which is stable and efficient to produce VLPs and modify the surface of VLPs as an alternative. Furthermore, we also provide two strategies of VLPs construction which have more than one structural protein, one is coinfection, and the other one is coexpression. The insect cell line derived from Trichoplusia ni has been used for the production of the commercial HPV L1-VLP vaccine. Of note, at least 7 additional recombinant VLPs that are isolated from insect cell systems are at different development stages as promising vaccine candidates.


Advantages & Our services

Leading Biology is pleased to tailor the most appropriate experimental scheme for our customs based on different research directions. Alternative expression systems are also available from which we can select the most appropriate choice to proceed:

  • VLP Production in E. coli System
  • VLP Production in Yeast Cell System
  • VLP Production in Plant System
  • VLP Production in Mammalian Cell System

We are committed to being the premier, research-intensive biotechnology company and are dedicated to providing leading solutions for old and new customers. We are always dedicated to the highest level of scientific excellence. Please feel free to inquire us for further discussions.


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Virus-like particles/Phage library expression system based

Cell-free expression system

Whole cell-based protein expression system


Why Leading Biology?

At Leading Biology, we custom protein purification design for every single protein to ensure the production and recovery rate as high as possible.


Working with us, you will get stability, and it means a reliable partner to help streamline your R&D process.


Working with us, you will get the guaranteed service to accommodate your requirements.

  • Innovative configurations of chromatography columns custom-tailored to each protein
  • Vigorous quality control system to ensure the required quality and reproducibility
  • Production capacity of up to tens of grams
  • Flexible scale-up protein production
  • Competitive price with fast turnaround time


Contact Information

Please obtain a quote before ordering, and refer to the quote number when you place an order.

Orders are typically confirmed within 12 hours.

Have a Question? Email us info@leadingbiology.com

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