Introduction

Although using the traditional antibody preparation method to prepare antibodies against membrane protein facing lots of difficulties, considering the overall time and technical difficulty, monoclonal antibody preparation for some simple structure membrane proteins is still an economical and effective way.

Service Package

Classic case (Twice Transmembrane antibody service)

Customer Provide: Protein information

Process:

1. Protein structure analysis, recombinant protein design and codon optimization.

2. gene synthesis, vector construction and protein expression.

3. Feeding and immunization of 2 rabbits/antigen (3 immunizations, 3 weeks).

4. Blood collection, ELISA test (parallel with immunization)

5. Purification, detection and delivery

Products: (each antigen, 2 rabbits)

1. 3-5mL negative serum.

2. 15-20mL positive serum, ELISA titer was above 1:60,000.

3. Complete lab report, remaining antigens.

Preparation time: 3-4 months

Successful case:

Publication: Nat Cell Biol.        

IF: 7.73

Title: Mitochondrial outer-membrane protein FUNDC1 mediates hypoxia-induced mitophagy in mammalian cells.

Name: Anti-fundc1 antibody

Species: Human

Type: Rabbit polyclonal antibody

HeLa cells were transfected with plasmids encoding FUNDC1–Myc for the indicated times and the mitochondrial (TIM23, TOM20, VDAC1), endoplasmic reticulum (calnexin) and Golgi marker (GM130) proteins were detected by western blotting.

Why Leading Biology?

We offer the one-stop phage display service:

·      Immune, naive or synthetic library construction

·      Pre-made library screening

·      Monoclonal detection

·      Antibody expression

·      Custom biopanning strategies

·      QC: to ensure the success of your projects, we perform documentation QC for all assays.

Our advantages

·      World-leading sequencing capacity: use state-of-the-art technologies, such as Timer Condon technology, nanopore platform, etc with library capacity from 10⁸ to over 10¹³ ;

·      Various phage display systems: provide the most appropriate phage display system (M13, T4 or T7) to meet our customers’ demands.

·       High affinity: from 10^-7 to 10^-9

·      Custom biopanning strategies: The experienced technical team will work together to customize our products so as to meet our customers’ different needs. Leading Biology also utilizes the novel biopanning strategies to select high-affinity binders or ligands which are able to recognize the naive targets. Our Naive human antibody library panning includes: Pre-made yeast display naive human antibody library & Pre-made phage display naive human Fab library.

·      Rich experience: Our technicians refine every detail of our processing to enable the efficiency and high-quality. With extensive experience in phage display, we have provided satisfying service to our global researchers.

Contact US

We are proud to be able to support global scientists by providing high-quality service in a rapid time-frame. Reach out to us and we will get back to you within 24 hours.

Get a free quote

Email: info@leadingbiology.com

Phone: 1-661-524-0262 (USA)